High Performance Liquid Chromatography:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Transfection:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Enzyme-linked Immunosorbent Assay:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Plasmid Preparation:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Agarose Gel Electrophoresis:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Reverse Transcription Polymerase Chain Reaction:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Mini Gene Assay:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Construct:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Quantitative Proteomics:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Comparison:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Control:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Electrophoresis:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Cotransfection:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Expressing:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Generated:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Luciferase:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Variant Assay:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Virus:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Recombinant:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Protease Inhibitor:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Saline:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Cryo-EM Sample Prep:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Inhibition:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Blocking Assay:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
Cloning:
Article Title: Supporting information to Hepatitis E virus infection remodels the mature tRNAome in macrophages to orchestrate NLRP3 inflammasome response
Article Snippet: BIOLOGY Sigma-Aldrich Cat# 50046-50G L-ASPARTIC ACID, BIOXTRA, >=99% (HPLC) Sigma-Aldrich Cat# A8949-25G TRANS-4-HYDROXY-L-PROLINE, 99+% Sigma-Aldrich Cat# H54409-2.5G L-LYSINE MONOHYDROCHLORIDE MEETS EP, JP& Sigma-Aldrich Cat# L8662-25G FuGENE® HD Transfection reagent Promega Cat# E2311 L-Arginine Bio-Connect BV Cat# 0219462725 L-Proline Sigma-Aldrich Cat# P5607-25G L-Histidine Sigma-Aldrich Cat# H8000-5G L-Isoleucine Sigma-Aldrich Cat# I2752-1G L-Leucine Sigma-Aldrich Cat# L8000-25G L-Methionine Sigma-Aldrich Cat# M9625-5G L-Phenylalanine Sigma-Aldrich Cat# P5482-25G L-Serine Sigma-Aldrich Cat# S4500-1G L-Threonine Sigma-Aldrich Cat# T8625-1G L-Tryptophan Sigma-Aldrich Cat# T0254-1G L-Tyrosine Sigma-Aldrich Cat# T8566-25G L-Valine Sigma-Aldrich Cat# V0500-1G L-Asparagine Sigma-Aldrich Cat# A4159-25G L-Cystine Sigma-Aldrich Cat# C7602-10MG Lipopolysaccharide (LPS) Sigma-Aldrich Cat# L6529 BAY11 7085 Santa Cruz Biotech Cat# sc-202490 MCC950 Invivogen Cat# inh-mcc Belnacasan (VX-765) Bio-Connect BV Cat# S2228 Critical commercial assays Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Bio-Techne Cat# DLB50 Macherey NucleoSpin RNA II Kit Bioke Cat# 740955.250 Experimental models: Cell lines THP-1 Erasmus University Medical Center N/A HL 60 Erasmus University Medical Center N/A
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplemented with 4% low IgG FBS), and plates were returned to the incubator.
Article Title: Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10EMBacY competent cells This paper N/A Chemicals, peptides, and recombinant proteins ERDRP-0519 MedChemExpress Cat# HY-102074 AS-136A TargetMol Cat# T62160 EDTA-free protease inhibitor cocktail APExBIO Cat# K1007 [α- 32 P]-GTP, 250 μCi Revvity Cat# BLU506H250UC Dithiothreitol (DTT) Yeasen Cat# 20311ES60 NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) Yeasen Cat# 10133ES03 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432-10 Urea Sangon Biotech Cat# A510907 Proteinase K NEW ENGLAND Biolabs Cat# P8107S DMSO Thermo Fisher Scientific Cat# 85190 Surfactant P20 Cytiva/GE Cat# BR100054 Phosphate Buffered Saline (PBS) Hyclone Cat# SH30256.01 TBE Buffer Yeasen Cat# 60144ES76 2X TBE-Urea Sample Buffer Sangon Biotech Cat# C506046 FuGENE® HD Transfection Reagent Promega Cat# E2311 1M HEPES Buffer (pH8.0) Coolaber Cat# SL6053 NaCl Sangon Biotech Cat# A501218 MgCl 2 Sigma-Aldrich Cat# M8266 Tris-HCl Sangon Biotech Cat# B548124 Tween 20 Sigma-Aldrich Cat# 93773 Triton X-100 Sigma-Aldrich Cat# T9284 Acryl/Bis 30% Solution (29:1) Sangon Biotech Cat# B546017 Deposited data Model of the MeV L-P complex in apo state This paper PDB: 9KNQ Model of the MeV L-P complex bound to ERDRP-0519 This paper PDB: 9KNT Model of the MeV L-P complex bound to AS-136A This paper PDB: 9KNV Model of the NiV L-P complex bound to ERDRP-0519 This paper PDB: 9KNZ Cryo-EM map of the MeV L-P complex in apo state This paper EMD-62459 Cryo-EM map of the MeV L-P complex bound to ERDRP-0519 This paper EMD-62461 Cryo-EM map of the MeV L-P complex bound to AS-136A This paper EMD-62463 Cryo-EM map of the NiV L-P complex bound to ERDRP-0519 This paper EMD-62467 Experimental models: Cell lines High-five cells (Trichoplusia ni) Thermo Fisher Scientific Cat# B85502 Sf9 cells (Spodoptera frugiperda) Sigma-Aldrich Cat# 89070101-1VL Oligonucleotides NiV leader RNA (5’- AUUUUCCCUUGUUUGGU -3’) GenScript Biotech N/A Recombinant DNA pFastBac1 GenScript Biotech N/A MeV-L (GenBank: M20865.1) in pFastBac1 vector This paper N/A (Continued on next page) ll OPEN ACCESS e1 Cell 188, 1–11.e1–e4, September 4, 2025 Please cite this article in press as: Wang et al., Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition, Cell (2025), https://doi.org/10.1016/j.cell.2025.06.017 Article
Article Title: From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation
Article Snippet: For minigene assays, transfections of HeLa cells were performed using FuGENE® HD Transfection Reagent (Promega) with 3μg of plasmid DNA in a 1:1 ratio according to the manufacturer’s recommendations.
Article Title: Age-associated defect in ADCC response to COVID-19 vaccine.
Article Snippet: Cells were then transiently transfected with 50 ng/well of a SARS-CoV-2 spike expression plasmid using Fugene® HD Transfection Reagent (Promega) and maintained under the same conditions for 48 h. On the day of the assay, the culture media was replaced with 25 μL of assay media (RPMI 1640 supplementedwith 4% low IgGFBS), and plates were returned to the incubator.
Article Title: A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GST Antibody Cytiva Cat# 27457701; RRID:AB_771432 GFP tag Monoclonal antibody (mouse) Proteintech AB_11182611; RRID:AB_11182611 RFPtrap magnetic agarose beads Proteintech AB_2631362; RRID:AB_2631362 Bacterial and virus strains E. coli XL-1 blue Agilent Cat# 200249 E. coli BL21 Gold (DE3) Agilent Cat# 230132 E. coli BL21 (DE3) Thermo ScientificTM Cat# EC0114 E. coli DH10bac Invitrogen Cat# 10592663 Chemicals, peptides, and recombinant proteins 3C-protease In house N/A Q5® High-Fidelity DNA Polymerase NEB Cat# M0491 10x Casein blocking buffer Sigma Aldrich Cat# B6429 FuGENE® HD Transfection Reagent Promega Cat# E2311 Aminoallyl-UTP-ATTO-680 Jena Bioscience Cat# NU-821-680 TURBOTM DNase Thermo Fisher Scientific Cat# AM2238 Terminal Deoxynucleotidyl Transferase Thermo ScientificTM Cat# EP0161 Critical commercial assays GSTrap FF Cytiva Cat# 17513101 HisTrap FF Cytiva Cat# 17525501 HiTrap Heparin HP Cytiva Cat# 17040701 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HiLoad 16/600 Superdex 200 pg Cytiva Cat# 28989335 Superdex 200 Increase 10/300 Cytiva Cat# 28990944 CM5 Series S Sensor Chips Cytiva Cat# 29104988 GST capture kit Cytiva Cat# BR100223 Amine Coupling kit Cytiva Cat# BR100050 InFusion® HD Cloning Kit Takara Cat# 639650 HiScribe® T7 High Yield RNA Synthesis Kit NEB Cat# E2040S Deposited data BMRB: 50940: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 Vaishali et al.30 https://doi.org/10.13018/BMR50940 BMRB: 52764: Drosophila melanogaster Staufen dsRBD3-4 backbone chemical shift assignments This study https://doi.org/10.13018/BMR52764 Experimental models: Cell lines Sf21 insect cells GibcoTM Cat# 11497013 High FiveTM Cells Invitrogen BTI-TN-5B1-4 Experimental models: Fly strains Tm1[eg9] (FBal0049223) Erdélyi et al. 33 N/A oskarGAL4/TM3, Sb BDSC BDSC Cat# 44242; RRID:BDSC_51324 emGFP-Tm1 1-334/CyO Dimitrova-Paternoga et al. 25 N/A emGFP-Tm1 1-334 M12/CyO This study N/A emGFP-Tm1 1-334 L93Y/CyO This study N/A (Continued on next page) 14 Cell Reports 44, 115906, July 22, 2025
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